교수소개
김형권 교수는 서울대학교 자연과학대학 동물학과에서 학사학위를 받았으며, 한국과학기술원(KAIST) 생물과학과에서 석사, 박사학위를 받았다. 한국생명공학연구원(KRIBB) 응용미생물연구실에서 연구원, 선임연구원, 책임연구원으로 근무했으며, 미국 국립보건원(NIH)에서 visiting fellow로 근무하였다. 2003년부터 가톨릭대학교 생명공학과에서 근무하고 있으며, 학과장, 입학처장, 성심대학원장으로 활동하였다. 연구 관심사는 미생물, 메타게놈, 유전체정보로부터 지질가수분해 효소 발굴, 다양한 숙주에서 재조합효소 생산, 효소의 산업적 특성 규명, 분자진화기법과 위치특이적 돌연변이 기법으로 효소 개량, 효소의 3차구조 및 도킹구조 모델링, 효소의 고정화, 효소 반응을 통한 항산화, 항균 지질소재 생산, 플렉서블 리포솜 개발 분야이다. 인도네시아 AJCUI대학, IPB대학과 인적, 물적 공동연구를 활발히 수행하고 있다. 현재 한국미생물생명공학회(KMB)에서 평의원 및 JMB/MBL 편집위원으로 활동하고 있으며, 생물촉매분과 위원장, 학술간사, 소식지 간사로 활동하였다. 또한, 한국미생물학회에서 대외협력위원장으로 활동하였고, 한국효소공학연구회에서 이사 및 감사로 활동하고 있다.
Professor Hyung-Kwoun Kim is a professor in the Dept. of Biotechnology at the Catholic University of Korea. He received his bachelor's degree from the Dept. of Zoology, College of Natural Sciences, Seoul National Univ., and his master's and doctoral degrees from the Dept. of Biological Sciences, KAIST. He worked as a researcher, senior researcher, and principal investigator at the Applied Microbiology Lab at the KRIBB, and as a visiting fellow at the NIH in USA. His main research interests include discovery of lipolytic enzymes from microbial, metagenome, and genomic information, production of recombinant enzymes in various hosts, and industrial characterization of enzymes. He is also researching enzyme improvement using molecular evolution techniques and site-directed mutagenesis techniques, as well as tertiary structure and docking structure modeling of enzymes. And his research field is in the areas of enzyme immobilization, production of antioxidant/antibacterial lipid materials through enzyme reactions, and flexible liposome development. He is actively carrying out joint research with AJCUI Univ. and IPB Univ. in Indonesia. He currently serves as a council member and editorial board member of JMB/MBL at the KMB, and has served as chairman of the biocatalysis section, academic secretary, and newsletter secretary.
최종학력
1996.08.20 | 한국과학기술원 | 생물과학과 | 이학박사
연구실적
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2024.06
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제34권 6호, pp.1356-1364
Synthesis of Isoamyl Fatty Acid Ester, a Flavor Compound, by Immobilized Rhodococcus Cutinase 34(6): 1356-1364 -
2024.02
| 교신저자
| ENZYME AND MICROBIAL TECHNOLOGY, 제173권
Production of octyl butyrate using psychrophilic mutant lipase from Croceibacter atlanticus LipCA lipase developed by a molecular evolution technique -
2023.12
| 공동저자
| JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY, 제21권 1호
Cloning and characterization of an acidic lipase from a lipolytic bacterium in tempeh -
2023.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제33권 2호, pp.268-276
Synthesis of Short-Chain Alkyl Butyrate through Esterification Reaction Using Immobilized<i> Rhodococcus</i> Cutinase and Analysis of Substrate Specificity through Molecular Docking -
2023.01
| 교신저자
| CHEMISTRY AND PHYSICS OF LIPIDS, 제250권
Development of skin-permeable flexible liposome using ergosterol esters containing unsaturated fatty acids -
2022.08
| 교신저자
| PROTEIN EXPRESSION AND PURIFICATION, 제195권
Functional production, characterization, and immobilization of a cold-adapted cutinase from Antarctic Rhodococcus sp. -
2022.05
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제32권 5호, pp.672-679
Production of 4-Ethyl Malate through Position-Specific Hydrolysis of Photobacterium lipolyticum M37 Lipase -
2021.06
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제31권 6호, pp.882-889
Increased mRNA Stability and Expression Level of Croceibacter atlanticus Lipase Gene Developed through Molecular Evolution Process -
2021.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제31권 2호, pp.317-326
Antioxidant and Antibacterial Activity of Caprylic Acid Vanillyl Ester Produced by Lipase-Mediated Transesterification -
2020.09
| 교신저자
| ENZYME AND MICROBIAL TECHNOLOGY, 제139권, pp.109581-109581
Antibacterial activity of emulsions containing unsaturated fatty acid ergosterol esters synthesized by lipase-mediated transesterification -
2020.02
| 교신저자
| ENZYME AND MICROBIAL TECHNOLOGY, 제133권
Lipase-mediated synthesis of ricinoleic acid vanillyl ester and evaluation of antioxidant and antibacterial activity -
2020.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제30권 2호, pp.216-225
Characterization of Novel Salt-Tolerant Esterase Isolated from the Marine Bacterium Alteromonas sp. 39-G1 -
2019.12
| 교신저자
| APPLIED BIOLOGICAL CHEMISTRY, 제62권 1호
Evaluation of antioxidant and antimicrobial activity of phenolic lipids produced by the transesterification of 4-hydroxyphenylacetic acid and triglycerides -
2019.03
| 교신저자
| APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 제187권 3호, pp.1046-1060
Characterization of organic solvent-tolerant lipolytic enzyme from Marinobacter lipolyticus isolated from the Antarctic Ocean -
2018.09
| 교신저자
| MICROBIOLOGY AND BIOTECHNOLOGY LETTERS, 제46권 3호, pp.234-243
Production, Immobilization, and Characterization of Croceibacter atlanticus Lipase Isolated from the Antarctic Ross Sea -
2017.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제27권 2호, pp.289-296
Cadaverine production by using cross-linked enzyme aggregate of escherichia coli lysine decarboxylase -
2016.11
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제133권, pp.475-481
Antioxidant and antibacterial activity of fatty acid vanillyl ester produced by Proteus vulgaris K80 lipase-mediated transesterification -
2016.10
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제26권 10호, pp.1708-1716
Effects of N-/C-terminal extra tags on the optimal reaction conditions, activity, and quaternary structure of bacillus thuringiensis glucose 1-dehydrogenase -
2016.09
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제26권 9호, pp.1579-1585
Antibacterial effect of fructose laurate synthesized by Candida antarctica B lipase-mediated transesterification -
2016.07
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제129권, pp.54-60
Production of cinnamoyl lipids using immobilized Proteus vulgaris K80 lipase and an evaluation of their antioxidant activity -
2016.05
| 교신저자
| APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 제179권 2호, pp.347-360
Production of Omega-3 Fatty Acid Ethyl Esters from Menhaden Oil Using Proteus vulgaris Lipase-Mediated One-Step Transesterification and Urea Complexation -
2015.11
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제25권 11호, pp.1810-1818
Production of (R)-Ethyl-4-Chloro-3-Hydroxybutanoate Using Saccharomyces cerevisiae YOL151W Reductase Immobilized onto Magnetic Microparticles -
2015.09
| 공동저자
| ACS CATALYSIS, 제5권 9호, pp.5016-5025
Recombinant Lipase Engineered with Amphipathic and Coiled-Coil Peptides -
2015.05
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제115권, pp.76-82
Transesterification reaction using Staphylococcus haemolyticus L62 lipase crosslinked on magnetic microparticles -
2015.04
| 교신저자
| JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, 제58권 2호, pp.195-202
One-step transesterification reaction using methanol-stable lipase for omega-3 fatty acid ethyl ester production -
2014.12
| 교신저자
| ENZYME AND MICROBIAL TECHNOLOGY, 제67권, pp.32-39
Transesterification of plant oils using Staphylococcus haemolyticus L62 lipase displayed on Escherichia coli cell surface using the OmpA signal peptide and EstA beta 8 anchoring motif -
2014.10
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제24권 10호, pp.1382-1388
Characterization of Proteus vulgaris K80 Lipase Immobilized on Amine-Terminated Magnetic Microparticles -
2013.12
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제97권 0호, pp.54-61
Cell surface display of Staphylococcus haemolyticus L62 lipase in Escherichia coli and its application as a whole cell biocatalyst for biodiesel production -
2013.10
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제23권 10호, pp.1395-1402
Development of a Bioconversion System Using Saccharomyces cerevisiae Reductase YOR120W and Bacillus subtilis Glucose Dehydrogenase for Chiral Alcohol Synthesis -
2013.09
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제23권 9호, pp.1221-1228
Expression and Biochemical Characterization of Cold-Adapted Lipases from Antarctic Bacillus pumilus Strains -
2013.05
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제23권, pp.661-667
Isolation and Biochemical Characterization of Bacillus pumilus Lipases from the Antarctic -
2013.02
| 공동저자
| JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, 제56권 1호, pp.55-62
Shewanella sp. Ke75 Esterase with Specificity for p-nitorphenyl Butyrate: Gene Cloning and Characterization -
2013.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제23권 2호, pp.218-224
Development of Saccharomyces cerevisiae reductase YOL151W mutants suitable for chiral alcohol synthesis using an NADH cofactor regeneration system -
2013.01
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제85-86권, pp.10-16
Biodiesel production using cross-linked Staphylococcus haemolyticus lipase immobilized on solid polymeric carriers -
2012.12
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제84 Special권 SI호, pp.15-21
Synthesis of a chiral alcohol using a rationally designed Saccharomyces cerevisiae reductase and a NADH cofactor regeneration system -
2012.12
| 교신저자
| JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 제114권 6호, pp.589-595
Gene cloning and catalytic characterization of cold-adapted lipase of Photobacterium sp. MA1-3 isolated from blood clam -
2012.09
| 공동저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제22권 9호, pp.1245-1252
Gene Cloning and Characterization of a Cold-Adapted Esterase from Acinetobacter venetianus V28 -
2012.01
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제22권 1호, pp.84-91
Characterization of Lipases from Staphylococcus aureus and Staphylococcus epidermidis Isolated from Human Facial Sebaceous Skin -
2011.12
| 공동저자
| JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 제57권 6호, pp.357-364
A novel cold-adapted esterase from Salinisphaera sp. P7-4: Gene cloning, overproduction, and characterization -
2011.11
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제21권 11호, pp.1159-1165
Transesterification Using the Cross-Linked Enzyme Aggregate of Photobacterium lipolyticum Lipase M37 -
2011.09
| 교신저자
| 한국미생물.생명공학회지, 제39권 3호, pp.238-244
Proteus vulgaris에서 유래한 리파아제의 고정화 및 바이오디젤 생산 -
2011.07
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제70권 3-4호, pp.114-118
Production of chiral compound using recombinant Escherichia coli cells co-expressing reductase and glucose dehydrogenase in an ionic liquid/water two phase system -
2010.09
| 교신저자
| BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 제1804권 9호, pp.1841-1849
Asymmetric synthesis of (S)-ethyl-4-chloro-3-hydroxy butanoate using a Saccharomyces cerevisiae reductase: Enantioselectivity and enzyme-substrate docking studies -
2010.09
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제20권 9호, pp.1300-1306
Enantioselective Bioconversion Using Escherichia coli Cells Expressing Saccharomyces cerevisiae Reductase and Bacillus subtilis Glucose Dehydrogenase -
2010.06
| 교신저자
| APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 제87권 1호, pp.185-193
Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity -
2010.02
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제20권, pp.325-331
Biotransformation of Amides to Acids Using a Co-Cross-Linked Enzyme Aggregate of Rhodococcus erythropolis Amidase -
2009.06
| 교신저자
| 한국미생물.생명공학회지, 제37권 2호, pp.118-124
메타게놈유래의 저온성 에스터라제 EM2L8의 효소적 특성과 이를 활용한 고지혈증 치료제 키랄소재의 생산 -
2009.06
| 교신저자
| JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 제107권, pp.599-604
Catalytic properties of a lipase from Photobacterium lipolyticum for biodiesel production containing a high methanol concentration -
2009.01
| 공동저자
| APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 제81권, pp.865-874
Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome -
2008.12
| 교신저자
| 한국미생물.생명공학회지, 제36권 4호, pp.299-306
비만 억제를 위한 췌장 리파아제 도메인에 대한 특이 난황항체의 개발 -
2008.11
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제55권, pp.157-163
Biochemical characterization of Rhodococcus erythropolis N ' 4 nitrile hydratase acting on 4-chloro-3-hydroxybutyronitrile -
2008.04
| 공동저자
| PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 제71권, pp.476-484
Structural basis for the cold adaptation of psychrophilic M37 lipase from Photobacterium lipolyticum -
2008.04
| 공동저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제18권, pp.616-623
Isolation and characterization of marine bacterial strain degrading fucoidan from Korean Undaria pinnatifida sporophylls -
2008.03
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제18권, pp.552-559
R-stereoselective amidase from Rhodococcus erythropolis no. 7 acting on 4-chloro-3-hydroxybutyramide -
2007.04
| 교신저자
| PROTEIN EXPRESSION AND PURIFICATION, 제52권, pp.340-347
Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome -
2007.04
| 공동저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제45권, pp.34-38
Enantioselective resolution of methyl 2-chloromandelate by Candida antarctica lipase A in a solvent-free transesterification reaction -
2006.09
| 교신저자
| 한국미생물.생명공학회지, 제34권 3호, pp.204-210
니트릴 분해효소 생산균인 Rhodococcus erythropolis의 발굴 및 효소 특성 연구 -
2006.04
| 교신저자
| APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 제70권, pp.321-326
New cold-adapted lipase from Photobacterium lipolyticum sp nov that is closely related to filamentous fungal lipases -
2006.03
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제16권, pp.408-413
Gene cloning of Streptomyces phospholipase D P821 suitable for synthesis of phosphatidylserine -
2006.01
| 교신저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제16권, pp.145-148
Expression and characterization of a new esterase cloned directly from Agrobacterium tumefaciens genome -
2005.03.01
| 교신저자
| 한국미생물.생명공학회지, 제33권 1호, pp.29-34
갯벌에서 분리된 Psychrobacter sp. S3균 유래의 저온성 리파제에 의한 트리글리세리드의 가수분해 특성 -
2005.02.01
| 공동저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제15권 1호, pp.155-160
A direct approach for finding functional lipolytic enzymes from the Paenibacillus polymyxa genome -
2004.12.24
| 공동저자
| , 제272권, pp.538-549
A novel lipase/chaperone pain from Balstonia sp. M1 : analysis of the folding interaction and evidence for gene loss in R.solanacearum -
2004.06.01
| 공동저자
| FEMS MICROBIOLOGY LETTERS, 제235권, pp.349-355
Sequence-based approach to finding functional lipases from microbial genome databases -
2004.03.01
| 공동저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제27권, pp.237-241
Occurrence of ofloxacin ester-enantioselective esterase from Bacillus niacini EM001 -
2003.12.01
| 공동저자
| JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 제13권 6호, pp.892-896
High-Level Expression and Secretion of Bacillus pumilus lipase B26 in Bacillus subtilis Chungkookjang -
2003.12.01
| 교신저자
| 한국미생물.생명공학회지, 제31권 4호, pp.311-321
세균성 리파제의 분자구조와 작용기작 -
2003.12.01
| 교신저자
| JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 제26권, pp.223-229
Nonionic detergent-induced activation of an esterase from Bacillus megaterium 20-1 -
2003.12.01
| 교신저자
| , 제24권, pp.151-162
Differential Calcium Effects on Lipases from Bacillus pumilus, Bacillus stearothermophilus, and Proteus vulgaris
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2011.01
| 공동
| 월드사이언스
미생물생물공학 -
2006.08.01
| 공동
| 라이프사이언스
산업응용 미생물학
-
2023.07.04
| 주발명자
| 가톨릭대학교 산학협력단
리파아제를 이용한 지방산 에르고스테롤 에스터 화합물의 효율적인 제조방법 및 이에 의해 제조된 지방산 에르고스테롤 에스터 화합물의 용도(Efficient method for preparing fatty acid ergosterol ester compound using lipase and use of ergosterol ester compound prepared thereby) -
2021.03.11
| 주발명자
| 가톨릭대학교 산학협력단
신규한 페놀 에스터 화합물 및 이의 용도(Novel phenol ester compound and uses thereof) -
2021.02.10
| 공동발명자
| 한국해양과학기술원
신규한 하이드록시페닐아세트산 유도체를 유효성분으로 함유하는 항산화 또는 항균용 조성물(Composition for Antioxidant or Antibacterial Containing Novel Hydroxyphenylacetic Acid Derivatives) -
2020.11.30
| 공동발명자
| 한국해양과학기술원
크로세이박터 아틀란티쿠스 유래 지질분해효소(Lipase from Croceibacter atlanticus) -
2020.06.25
| 공동발명자
| 한국해양과학기술원
해양 박테리아 유래 에스터레이즈 변이 효소(Variants of Esterase Isolated from the Marine Bacterium) -
2020.03.31
| 공동발명자
| 한국해양과학기술원
마리노박터 리포리티쿠스 유래 지질분해효소(Lipase from Marinobacter lipolyticus) -
2016.02.16
| 공동발명자
| 한국해양과학기술원
남극 유래 바실러스 푸밀러스 리파아제(Lipase of Bacillus pumilus from the Antarctic) -
2014.08.29
| 주발명자
| 가톨릭대학교 산학협력단
스타필러코커스 해모라이티쿠스 L62 리파아제의 세포 표면 발현과 트랜스에스테르화 생물 전환 공정의 응용(Cell Surface Display of Staphylococcus haemolyticus L62 Lipase and Its Application for Transesterificatin Reaction) -
2014.04.29
| 주발명자
| 가톨릭대학교 산학협력단
스타필러코커스 해모라이티쿠스 리파아제 엘62의 고정화 방법과 이를 이용한 지방산 에스테르 제조 방법(Method of Immobilization of Staphylococcus haemolyticus L62 Lipase and Its Application for Fatty Acid Ester Production) -
2014.04.03
| 공동발명자
| 한국해양과학기술원
심해 해저에서 분리된 에스터라제 KTL 4(Esterases KTL4 Isolated from Deep Sea Sediment) -
2011.11.28
| 주발명자
| 가톨릭대학교 산학협력단
생물학적 촉매를 고정화하는 방법 및 이의 응용(Method for Immobilizing Biocatalysts and Application Thereof) -
2011.11.01
| 공동발명자
| 주식회사 단바이오텍
비만과 고지혈증 예방 및 치료를 위한 특이 난황항체를 포함하는 조성물(Composition for preventing and treating obesity and hyperlipidemia comprising specific egg yolk antibody)
-
2016.01.06
| 주발명자
| ㈜세포바이오
트립신, 콜라게나제 및 디스파제를 포함하는 세포 탈착용 조성물
한국생명공학연구원 응용미생물연구실 (1989년-2003년) : 연구원, 선임연구원, 책임연구원 미국 NIH, NHLBI (1997년-1999년) : Visiting Fellow 한국미생물생명공학회 : 평의원, JMB 편집위원, MBL 편집위원, 학술간사, 소식지 편집간사, 감사, 효소촉매분과 위원장 가톨릭대학교 : 생명공학과 교수, 학과장, 입학처장, 성심대학원장
게놈과 리피돔 정보를 이용한 기능성 지질의 효소합성기술 개발 : 한국연구재단/중견연구자 지원사업 (2014-2017) 리파아제의 비수계 효소반응을 이용한 항산화, 항균 페놀리피드 합성 : 한국연구재단/이공학개인기초연구지원사업 (2018-2021) 리파아제 반응을 이용한 멀티 기능성 리피드 신소재 합성과 프렉서블 리포솜 개발 : 한국연구재단/이공학개인기초연구지원사업 (2022-2025) 극지 다기능 저온성 리파아제 효소 발굴과 기능성 소재 합성 : 극지연구소 과제 (2023-2025)